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Secondary Antibodies

Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies (44,275)
Mouse Secondary Antibodies (14,507)
Rabbit Secondary Antibodies (6,327)
Donkey Secondary Antibodies (4,321)
Rat Secondary Antibodies (1,240)
Sheep Secondary Antibodies (703)
Chicken Secondary Antibodies (403)
Bovine Secondary Antibodies (257)
Horse Secondary Antibodies (76)
Pig Secondary Antibodies (76)
Alpaca Secondary Antibodies (62)
Hamster Secondary Antibodies (55)
Biosimilar Secondary Antibodies (9)
Human Secondary Antibodies (7)
Duck Secondary Antibodies (3)

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1,411 – 1,425 of 72,126 results
1,411

Donkey anti-Goat IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles.
Target Species Goat
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.50mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins
1,412

Invitrogen™ F(ab')2-Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, HRP

F(ab')2-Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Rat
Host Species Donkey
Conjugate HRP
Applications ELISA,Immunohistochemistry,Western Blot
Form Lyophilized
Isotype IgG
Concentration 1 mg/mL
Antigen Rat IgG (H+L) Highly Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity Chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.1% ProClin 150; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,414

Invitrogen™ Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DSB-X Biotin

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage 4°C, store in dark
Target Species Goat
Host Species Donkey
Conjugate DSB-X Biotin
Applications Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 2 mg/mL
Antigen Goat IgG (H+L) Cross-Adsorbed
Regulatory Status RUO
Purification Method Purified
Product Type Secondary Antibody
Formulation PBS with 5mM sodium azide; pH 7.5
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,415

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Rabbit
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunocytochemistry/Immunofluorescence, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Rabbit IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins
1,416

Donkey anti-Goat IgG (H+L) Secondary Antibody, Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 8 publications

Content And Storage Store at 4C. Do not freeze.
Target Species Goat
Host Species Donkey
Conjugate Unconjugated
Applications Adhesion Activation,ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Frozen),Immunohistochemistry (Paraffin),Western Blot
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration 1.0 mg/mL
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot 1:1, ELISA 1:1, Immunohistochemistry 1:200-1:2000, Immunocytochemistry/Immunofluorescence 1:200 - 1:2, Immunohistochemistry-Paraffin 1:200 - 1:2000, Immunohistochemistry-Frozen 1:200-1:2000, Adhesion Activation
Classification Polyclonal
Immunogen Goat IgG-heavy and light chain
Primary or Secondary Secondary
Test Specificity By immunoelectrophoresis and ELISA this reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No was detected against non-immunoglobulin serum proteins. This may cross react with IgG from other species.
1,417

Donkey anti-Mouse IgG (H+L) Secondary Antibody, DyLight 350, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Mouse
Host Species Donkey
Conjugate DyLight 350
Applications ELISA,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Formulation 50mM Sodium Borate
Classification Polyclonal
Immunogen Donkey anti-Mouse IgG (H+L) Secondary Antibody (Pre-adsorbed) was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Primary or Secondary Secondary
1,418

Donkey anti-Goat IgG (H+L) Secondary Antibody, PE (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody has been used in 3 publications

Content And Storage Store at 4C in the dark.
Target Species Goat
Host Species Donkey
Conjugate PE
Applications Dot Blot,Flow Cytometry,Immunocytochemistry/Immunofluorescence,Western Blot
Isotype IgG
Concentration 0.5 mg/mL
Antigen IgG (H+L)
Gene Symbols IGHG1
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Western Blot, Flow Cytometry 1:100 - 1:250, Immunocytochemistry/Immunofluorescence 1:100 - 1:250, Dot Blot
Classification Polyclonal
Immunogen Goat IgG whole molecule
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
1,419

Donkey anti-Mouse IgG (H+L) Secondary Antibody, FITC, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Mouse
Host Species Donkey
Conjugate Fluorescein
Applications Flow Cytometry,Immunocytochemistry/Immunofluorescence,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:1000 - 1:10000, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:500 - 1:2500
Classification Polyclonal
Immunogen Mouse IgG whole molecule
Primary or Secondary Secondary
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Mouse IgG and Mouse Serum.
1,420

Donkey anti-Sheep IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C in the dark.
Target Species Sheep
Host Species Donkey
Conjugate Fluorescein
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity Purified
Dilution Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Reconstitution Reconstitute with 1.0 ml deionized water (or equivalent).
Primary or Secondary Secondary
Test Specificity This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbits and Rat Serum Proteins.
1,421

Invitrogen™ Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Biotin

Donkey Polyclonal Secondary Antibody

ENCOMPASS_PREFERRED
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target Species Rat
Host Species Donkey
Conjugate Biotin
Applications ELISA,Flow Cytometry,Western Blot,Immunocytochemistry
Form Liquid
Isotype IgG
Concentration 1.5 mg/mL
Antigen Rat IgG (H+L) Highly Cross-Adsorbed
Regulatory Status RUO
Purification Method Affinity chromatography
Product Type Secondary Antibody
Formulation PBS with 1% BSA and 0.05% sodium azide; pH 7.2
Immunogen Gamma Immunoglobins Heavy and Light chains.
Classification Polyclonal
Primary or Secondary Secondary
1,422

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4C. Do not freeze.
Target Species Sheep
Host Species Donkey
Conjugate Alkaline Phosphatase (AP)
Applications ELISA,Immunohistochemistry,Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Multi-step
Dilution Western Blot 1:1000 - 1:4000, ELISA 1:2000 - 1:8000, Immunohistochemistry 1:200 - 1:1000
Classification Polyclonal
Immunogen Donkey anti-Sheep IgG (H+L) Secondary Antibody [Alkaline Phosphatase] was produced by repeated immunization with Sheep IgG whole molecule in donkey.
Primary or Secondary Secondary
Test Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Sheep IgG and Sheep Serum.
1,423

Donkey anti-Rabbit IgG (H+L) Secondary Antibody, PerCP, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C in the dark.
Target Species Rabbit
Host Species Donkey
Conjugate PerCP
Applications ELISA,Immunocytochemistry/Immunofluorescence,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Formulation PBS
Classification Polyclonal
Immunogen This Donkey anti-Rabbit IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against rabbit IgG.
Primary or Secondary Secondary
1,424

Donkey anti-Sheep IgG (H+L) Secondary Antibody, Rhodamine, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Target Species Sheep
Host Species Donkey
Conjugate Rhodamine
Applications Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Immunohistochemistry
Form Purified
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Immunogen affinity purified
Dilution Flow Cytometry 1:500 - 1:2500, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
Classification Polyclonal
Immunogen Sheep IgG whole molecule
Primary or Secondary Secondary
Test Specificity Sheep IgG (H and L)
1,425

Donkey anti-Goat IgG (H+L) Secondary Antibody, DyLight 594, Novus Biologicals™
SDP

Donkey Polyclonal Antibody

Content And Storage Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage.
Target Species Goat
Host Species Donkey
Conjugate DyLight 594
Applications Flow Cytometry,Immunomicroscopy
Isotype IgG
Research Discipline Adaptive Immunity, Immunology
Concentration LYOPH
Antigen IgG (H+L)
Regulatory Status RUO
Purification Method Affinity purified
Dilution Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000
Classification Polyclonal
Immunogen Purified Goat IgG (H&L)
Reconstitution Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Primary or Secondary Secondary
Test Specificity Based on IEP, this antibody reacts with heavy gamma chains on goat IgG and light chains on all goat immunoglobulins